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A practical method for the preparation of total DNA from filamentous fungi

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Abstract

Most methods of DNA preparation from fungi are time-consuming due to the need to first make protoplasts, expensive for chemicals such as cesium chloride, or suitable only for small scale preparations. We have developed a simple method for total DNA preparation, yielding a product of quality suitable for restriction digestion and library construction.

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BORGES, M. I., AZEVEDO, M. O., BONATELLI, R., JR., FELIPE, M. S. & ASTOLFI- FILHO, S., (1990) “A practical method for the preparation of total DNA from filamentous fungi”, Fungal Genetics Reports 37(1). doi: https://doi.org/10.4148/1941-4765.1466

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Published on
1990-01-01