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Expression and visualization of Green Fluorescent Protein (GFP) in Neurospora crassa

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Abstract

We report the first successful imaging of GFP expression in Neurospora crassa. GFP was expressed under the control of the heterologous ToxA promoter from Pyrenophora tritici-repentis in transformants carrying multiple or single copies of the GFP construct. GFP was also detected in ascospores but not during earlier stages of the sexual cycle.

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Freitag, M., Ciuffetti, L. M. & Selker, E. U., (2001) “Expression and visualization of Green Fluorescent Protein (GFP) in Neurospora crassa”, Fungal Genetics Reports 48(1), 15-19. doi: https://doi.org/10.4148/1941-4765.1172

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Published on
2001-09-01