Abstract
We report the utilization of Discosoma sp. red fluorescent protein DsRed to visualize peroxisomes in the filamentous ascomycete Sordaria macrospora. To achieve labeling of peroxisomes, DsRed was fused to a serine-lysine-leucine tag (SKL). Expression of theDsRed-SKL fusion gene under the control of the Aspergillus nidulans gpd-promoter led to protein import of DsRed into peroxisomes. In this study, we describe our results concerning the construction as well as the application of vector pDsRed-SKL.
How to Cite:
Elleuche, S. & Pöggeler, S., (2008) “Visualization of peroxisomes via SKL-tagged DsRed protein in Sordaria macrospora”, Fungal Genetics Reports 55(1), 9-12. doi: https://doi.org/10.4148/1941-4765.1083
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