Abstract
The pOKE103 plasmid can be used to transform Neurospora crassa using the pan-2 gene (NCU10048.3) as a selective marker. We mapped the pan-2 fragment to the right arm of LG VI, contig 4, and found that it carries a pan-2 linked gene, atg-12 (NCU10049.3). We report the removal of atg-12 to produce an improved plasmid, pOKE104.
How to Cite:
Low, W. & Jedd, G., (2008) “An improved plasmid for transformation of Neurospora crassa using the pan-2 gene as a selectable marker”, Fungal Genetics Reports 55(1), 44-45. doi: https://doi.org/10.4148/1941-4765.1091
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