Abstract
Disruption of the nonhomologous end-joining (NHEJ) pathway has been shown to increase the efficiency of transgene integration into targeted genomic locations of Neurospora crassa and other fungi. Here, we report that a similar phenomenon occurs in a second Neurospora species: N. sitophila. Specifically, we show that deletion of N. sitophila mus-51 increases the efficiency of targeted-transgene integration, presumably by disrupting NHEJ. Researchers interested in obtaining the N. sitophila mus-51∆ strains described in this study can obtain them from the Fungal Genetics Stock Center (FGSC, Kansas State University, Manhattan, KS).
Keywords: Neurospora, NHEJ, nourseothricin, sitophila, transformation
How to Cite:
Rhoades, N. A., Webber, E. K. & Hammond, T. M., (2020) “A Nonhomologous End-Joining Mutant for Neurospora sitophila Research”, Fungal Genetics Reports 64(1). doi: https://doi.org/10.4148/1941-4765.2172
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