Abstract
The conventional approach for generating gene replacement constructs involves several sequence-specific cloning steps and is time-consuming. A ligation-PCR approach was developed to efficiently generate gene replacement constructs. Two vectors useful for this ligation-PCR approach and another vector suitable for improving the efficiency of knockout mutant screens were constructed.
How to Cite:
Zhao, X., Xue, C., Kim, Y. & Xu, J., (2004) “A Ligation-PCR Approach for Generating Gene Replacement Constructs in Magnaporthe grisea”, Fungal Genetics Reports 51(1), 17-18. doi: https://doi.org/10.4148/1941-4765.1137
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