Abstract
Here we report the construction and application of a range of expression plasmids designed to facilitate live-cell imaging of F-actin dynamics in filamentous fungi simultaneously with other, preferably GFP-tagged fusion proteins. Pros and cons of the use of three different red fluorescent proteins (RFPs), two different promoters and three different selection markers are addressed.
How to Cite:
Lichius, A. & Read, N. D., (2010) “A versatile set of Lifeact-RFP expression plasmids for live-cell imaging of F-actin in filamentous fungi”, Fungal Genetics Reports 57(1), 8-14. doi: https://doi.org/10.4148/1941-4765.1070
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