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Production of tyrosinase defective mutants of Neurospora crassa

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Abstract

We have produced mutants defective in tyrosinase activity using the RIP procedure (Selker et al. 1987 Cell 51:741-752, Marathe et al. 1990 Mol. Cell. Biol. 10:2638-2644). The tyrosinase gene has been shown to be a useful reporter in gene expression experiments (Kothe et al. 1993 FGN 40: 43-45). The availability of tyrosinase mutants will assist in these studies and will also be of use for future protein engineering experiments on tyrosinase (Fuentes et al. 1993 FGN 40:38-39)

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Fuentes, A. M., Connerton, I. & Free, S. J., (1994) “Production of tyrosinase defective mutants of Neurospora crassa”, Fungal Genetics Reports 41(1), 38-39. doi: https://doi.org/10.4148/1941-4765.1371

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Published on
1994-01-01