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An effect of homology length on gene disruption in Neurospora crassa

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Abstract

For targeted gene disruption in wild-type Neurospora crassa, 1000-bp of homologous sequences on either side of the cassette used for disruption is sufficient to give more than 10 % homologous recombination. We report here that varying the length of homology on each side seems to have different effects on the homologous recombination frequency.

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Nakanishi, Y., Ishii, C. & Inoue, H., (2005) “An effect of homology length on gene disruption in Neurospora crassa”, Fungal Genetics Reports 52(1), 5-6. doi: https://doi.org/10.4148/1941-4765.1120

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Published on
2005-12-01