Abstract
We report the expression of Discosoma red fluorescent protein (RFP) and RFP fusion proteins in Neurospora crassa. RFP was expressed under the control of the Neurospora ccg-1 promoter in transformants with single copies integrated at the his-3 locus by gene targeting. Because this RFP gene, tdimer2(12), contains a 677 bp direct tandem repeat of dsRed, RFP constructs underwent RIP at high frequency in rid+ strains. Fusion proteins of RFP to the amino terminus of Neurospora heterochromatin protein 1 (HP1) were localized to heterochromatic foci in Neurospora nuclei, consistent with prior findings with carboxy-terminal HP1-GFP fusion proteins.
How to Cite:
Freitag, M. & Selker, E. U., (2005) “Expression and Visualization of Red Fluorescent Protein (RFP) in Neurospora crassa”, Fungal Genetics Reports 52(1), 14-17. doi: https://doi.org/10.4148/1941-4765.1124
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