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Reliable PCR amplification from Neurospora crassa genomic DNA obtained from conidia

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Abstract

Boil-mediated lysis of Neurospora conidia (Boil-prep) is an extremely rapid, convenient and useful technique to obtain sufficient genomic DNA template for PCR amplification. We routinely use this technique for screening molecular markers, sequencing, and preliminary confirmation of transformants.

How to Cite:

Henderson, S. T., Eariss, G. A. & Catcheside, D. E., (2005) “Reliable PCR amplification from Neurospora crassa genomic DNA obtained from conidia”, Fungal Genetics Reports 52(1), 24. doi: https://doi.org/10.4148/1941-4765.1126

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Published on
2005-12-01