Abstract
We report the construction of pRAUW122, a plasmid capable of directing the integration of gene-constructs by gene replacement at the his-3locus of Neurospora crassa. Gene targeting at his-3 is selected by growing the transformants in histidine-free medium. The elimination of a second selectable marker (Bmlr), flanking the gene construct, is detected by screening for benomyl sensitivity of the his-3+ transformants. Therefore, his-3+ Bmls transformants have a high probability of having arisen by the desired gene replacement event.
How to Cite:
Aramayo, R. & Metzenberg, R. L., (1996) “Gene replacements at the his-3 locus of Neurospora crassa”, Fungal Genetics Reports 43(1), 9-13. doi: https://doi.org/10.4148/1941-4765.1299
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