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Methods for extracting markers from Spore killer strains of Neurospora crassa

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  • Methods for extracting markers from Spore killer strains of Neurospora crassa

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    Methods for extracting markers from Spore killer strains of Neurospora crassa

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Abstract

A mutant gene is most simply transferred from killer to nonkiller by crossing a marked killer strain x Sk sensitive acr-2R, plating ascospores on medium supplemented with acriflavine, and recovering mutant progeny among the acriflavine-resistant colonies, which are nonkillers that presumably originated from rare heterozygous or heterokaryotic ascospores.

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Perkins, D. D., (1996) “Methods for extracting markers from Spore killer strains of Neurospora crassa”, Fungal Genetics Reports 43(1), 43-44. doi: https://doi.org/10.4148/1941-4765.1315

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Published on
1996-07-25