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Protocol for the Electroporation of Neurospora Spheroplasts

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Abstract

A protocol for electroporating Neurospora strains is described. This protocol uses spheroplasts for transformation rather than germinated conidia with enzyme-weakened walls. High efficiency transformation is obtained with this system even with batches of Novozyme234 which worked poorly in the PEG based protocol.

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Kothe, G. O. & Free, S. J., (1996) “Protocol for the Electroporation of Neurospora Spheroplasts”, Fungal Genetics Reports 43(1), 31-32. doi: https://doi.org/10.4148/1941-4765.1309

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Published on
1996-07-25