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Construction of two Neurospora crassa Lambda ZapII-cDNA libraries

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Abstract

In the presence of the anti-microtubule agent benomyl, N. crassa synthesizes and secretes a major 60 kDa protein in the culture medium. This protein is absent in untreated cells (Rossier et al. 1989. Eur. J. Cell Biol. 50:333-339). To clone the gene coding for the 60 kDa protein and to unravel its function, we have constructed a cDNA library from benomyl-treated cells ("benomyl" cDNA library). A second cDNA library was constructed from untreated cells in order to clone genes coding for cytoskeletal proteins ("standard" cDNA library).

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Hoang-Van, K., Rossier, C. & Turian, G., (1991) “Construction of two Neurospora crassa Lambda ZapII-cDNA libraries”, Fungal Genetics Reports 38(1), 73-74. doi: https://doi.org/10.4148/1941-4765.1452

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Published on
1991-01-01