Abstract
The qa-2+ gene has been widely used as a selectable marker in Neurospora transformations (Akins and Lambowitz 1985. Mol. Cell. Biol. 5:2272-2278). A plasmid (pMSN1) has been constructed to facilitate the cloning of selected genes into a qa-2+ vector. The system takes advantage of the blue/white screening possible when cloned fragments are inserted into the E. coli lacZ (beta-galactosidase) gene, and also facilitates subsequent sequencing of the cloned genes.
How to Cite:
Nelson, M. A. & Metzenberg, R. L., (1992) “A qa2+-pGEM vector for Neurospora transformations”, Fungal Genetics Reports 39(1), 50-60. doi: https://doi.org/10.4148/1941-4765.1437
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